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European Surgery
Erschienen in:

01.12.2013 | original article

Impact of pneumoperitoneum on collagen I expression in vitro

verfasst von: T. F. Ulmer, MD, Dr. M. Binnebösel, A. Mossdorf, Prof. Dr. U. P. Neumann, Dr. R. Rosch

Erschienen in: European Surgery | Ausgabe 6/2013

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Summary Background Methods Results Conclusions

There is little and contradictory data about the impact of laparoscopy on wound healing. In this experimental study, gas-related effects of pneumoperitoneum on collagen I mRNA synthesis were analysed separately in fibroblast cultures in vitro.
3T3 mouse fibroblasts were incubated for 1 h with either CO2 or helium (gas flow: 1 l/min, pressure: 12 mmHg). Control fibroblasts were exposed to room air for 1 h. Hereafter, expression of collagen type IA1 was analysed by real-time PCR.
Expression of collagen type IA1 analysed was 0.7 times lower after incubation with CO2 when compared with controls. After helium insufflation, collagen type IA1 expression showed a 1.1-fold increase.
The observed gas-related influence on collagen synthesis might give evidence of a potential effect of laparoscopy on intra-abdominal wound healing.
Vorheriger Artikel The effect of harmonic scalpel, electrocautery, and scalpel use on early wound complications after modified radical mastectomy
Nächster Artikel One hundred double adenomas in primary hyperparathyroidism
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Metadaten
Titel
Impact of pneumoperitoneum on collagen I expression in vitro
verfasst von
T. F. Ulmer, MD
Dr. M. Binnebösel
A. Mossdorf
Prof. Dr. U. P. Neumann
Dr. R. Rosch
Publikationsdatum
01.12.2013
Verlag
Springer Vienna
Erschienen in
European Surgery / Ausgabe 6/2013
Print ISSN: 1682-8631
Elektronische ISSN: 1682-4016
DOI
https://doi.org/10.1007/s10353-013-0232-x

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